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>> January 30, 2011

The CHROMacademy Essential Guide to…. Hydrophilic Interaction Chromatography (HILIC) and Related Techniques (Part 1)Understanding HILIC and Related Techniques:
Just what is HILIC?
Fundamental HILIC and Mixed Mode
Retention Mechanisms
Optimizing Solvent Strength & Retention
The influence of pH
Buffers or additives or both?
Stationary Phases for HILIC and Mixed Mode Chromatography
pH, eluotropic and buffer strength
Gradients
Practical Issues / Column Equilibration
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